human bronchial epithelial-like hbe cells cl-0346  (Procell Inc)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Luteolin Inhibits the Biofilm Formation and Cytotoxicity of Methicillin-Resistant Staphylococcus aureus via Decreasing Bacterial Toxin Synthesis

doi: 10.1155/2022/4476339

Figure Lengend Snippet: Luteolin decreased the cytotoxicity of MRSA, and downregulated the expression of the hemolysin and hlaA genes in MRSA (a) Human bronchial epithelial cells were infected with luteolin-treated MRSA N315, and then the viability of human bronchial epithelial cells was measured using the CCK-8 assay. (b) Expressions of α -hemolysin and δ -hemolysin in luteolin-treated MRSA N315 were evaluated by western blot. (c) Level of hlaA in luteolin-treated MRSA N315 was detected using RT-PCR, and 16S was used as an endogenous control. ∗∗∗ P < 0.001, vs. Blank; ## P < 0.01, ### P < 0.001 vs. Control. (MRSA: methicillin-resistant Staphylococcus aureus , CCK-8: Cell Counting Kit-8, 16S: 16S rRNA, a -hemolysin: alpha hemolysin, d -hemolysin: delta hemolysin, RT-PCR: Real-Time PCR).

Article Snippet: Human bronchial epithelial cells (HBEs; CL-0346) were purchased from Procell (Wuhan, China) and grown in the specific cell medium (CM-0346, Procell, Wuhan, China).

Techniques: Expressing, Infection, CCK-8 Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control, Cell Counting, Real-time Polymerase Chain Reaction